Granuphilin c2a

Author: yozh

August undefined, 2024

WebGranuphilin C2A displays a lateral diffusion constant comparable to other C2 domains. However, the diffusion of the granuphilin C2AB tandem on the same membrane appears to be slower; suggesting substantial PS contacts for the C2B domain within the C2AB tandem. This effect represents a weak WebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before exocytosis. Slp-4 binds vesicular Rab proteins via an N-terminal …

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WebMay 31, 2024 · Synaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma membrane before … WebJan 1, 2024 · The results of an in vitro binding study indicated that the C2A domain of Slp3 and Slp5 functions as an atypical Ca 2+-dependent phospholipid-binding domain ... Slp4/granuphilin was originally identified as a rabphilin-like protein associated with insulin granules in pancreatic β cells (Wang et al. 1999). photo album with writing space refill

SYTL4 (synaptotagmin like 4)

WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2. 7: 24184645: 2014: The C2 domains of granuphilin are high-affinity sensors for plasma membrane lipids. 5: 27032672: 2016: Granuphilin exclusively mediates functional granule docking to the plasma membrane. 4: WebSep 1, 2014 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P 2]. The goal of this study was to determine membrane-binding mechanisms, affinities, and kinetics of both granuphilin C2 domains using fluorescence spectroscopic techniques. how does anytime fitness key work

Granuphilin C2A Domain as a Coincidence Detector for …

Multivalent lipid targeting by the calcium-independent …

WebFeb 11, 2024 · Seventy percent of the world’s internet traffic passes through all of that fiber. That’s why Ashburn is known as Data Center Alley. The Silicon Valley of the east. The … WebApr 1, 2016 · Furthermore, granuphilin forms a 180-nm cluster at the site of each docked granule, along with granuphilin-interacting Rab27a and Munc18-1 clusters. These … how does aortic stenosis cause heart failureWebNov 25, 2005 · Deletion analysis showed that the syntaxin-2/3 (or Munc18-1/2)-binding site is a linker domain of Slp4-a (amino acid residues 144-354), a previously uncharacterized region located between the N-terminal Rab27A binding domain and the C2A domain. We also found that the Slp4-a.syntaxin-2 complex is actually present in rat parotid glands … how does apay work

"WebOct 12, 2006 · Like granuphilin toward insulin granules, exophilin4 promotes the targeting of glucagon granules to the plasma membrane. Although the interaction of granuphilin with syntaxin-1a is critical for the targeting activity, exophilin4 does this primarily through the afﬁnity of its C2A domain toward the plasma membrane " - Granuphilin c2a

Granuphilin c2a

Weband the C2A domains of rabphilins (16). The PIP 2 binding site of the Slp-4 C2A domain was previously reported to center on this cluster (19). Notably, a large region around the cluster contains other basic residues that impart a positively charged electrostatic surface (Fig. 1). The Slp-4 C2A domain plays a critical role in targeting its WebMay 29, 2024 · Multivalent lipid targeting by the calcium-independent C2A domain of Slp-4/granuphilin Aml A Alnaas 1, Abena Watson-Siriboe 1, Sherleen Tran , Mikias Negussie, Jack A. Henderson , J. Ryan Osterberg 1, Nara Lee Chon , Julianna Oviedo,1 Tatyana Lyakhova1, Cole Michel2, Nichole Reisdorph 2, Richard Reisdorph , Colin T. Shearn3, …

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WebNov 1, 2013 · Granuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of … WebCorvallis Veterinary Hospital. Jul 2007 - Sep 20092 years 3 months. 1543 NW 9th St. Corvallis, OR 97330. As part of a small veterinary clinic I gained proficiency in a diverse set of skills ...

WebFeb 1, 2015 · The recognition of granuphilin C2A to PI(4,5)P2 within lipid bilayer was analyzed by NMR, using nanodisc system. Abstract. Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2) is a key player in regulating the process of excytosis, including insulin secretion. Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin ... WebJan 1, 2024 · The C2A domain of Slp-4 is known to drive this protein's ability to bind cellular PMs primarily via interaction with PIP 2 (12, 13).However, the protein retains an affinity for membranes containing the anionic lipids PS and/or PI, even in the absence of PIP 2 ().Here, we set out to discern the contributions of PIP 2 and background anionic lipid binding to …

WebThe C2A domain plays key roles in this process through interaction with PI (4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A … WebInsights into the molecular recognition of the granuphilin C2A domain with PI(4,5)P2 MLA Chanjuan Wan, et al. "Insights Into the Molecular Recognition of the Granuphilin C2a …

WebNov 5, 1990 · Granuphilin, a tandem C2 domain containing protein, mediates the docking of insulin granules onto plasma membrane. The C2A domain plays key roles in this process through interaction with PI(4,5)P2. In this study, we have investigated the molecular recognition mechanism of granuphilin-C2A domain to PI(4,5)P2 head group, and further …

WebJan 13, 2015 · Another example is the study of the interaction of granuphilin-C2A domain with phosphatidylinositol 4, 5-bisphosphate [PI(4, 5)P 2 ] head group and PI(4, 5)P 2 … how does ap poll workWebFeb 15, 2006 · Synaptotagmin-like protein 4-a (Slp4-a)/granuphilin-a is specifically localized on dense-core vesicles in certain neuroendocrine cells and negatively controls dense-core vesicle exocytosis through specific interaction with Rab27A. However, the precise molecular mechanism of its inhibitory effect on exocytosis has never been … how does apa differ from mlaWebGranuphilin contains two C2 domains, C2A and C2B, that interact with the plasma membrane lipid phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P2]. The goal of this … how does apa reference page lookWebSynaptotagmin-like protein 4 (Slp-4), also known as granuphilin, is a Rab effector responsible for docking secretory vesicles to the plasma … how does ap class workWebC2 Education is the fastest-growing test prep and tutoring center in the country. Use our Center Locator to help find a center near you and get started! how does apartment rental insurance workWebusing site-directed mutagenesis and NMR. Overall, granuphilin demonstrates the capability to bind to background anionic lipids and phosphoinositides inde-pendently, but the presence of both lipids greatly increases the binding afﬁnity of this protein. This suggests that granuphilin may serve as a coincidence de- photo albums 4x6 amazonNMR analysis of the granuphilin-C2A domain interacting with PI(4,5)P2-nanodisc demonstrates that, besides residues within the concave groove that are responsible for PI(4,5)P2 head group binding, the strand β5 was also affected. This region may be involved in direct binding to membrane and/or undergo conformation changes upon membrane binding. photo albums 4x6 3 ring