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Peasy®-t3 cloning vector

WebMar 30, 2024 · Vectors, Strains, Cell Lines and Reagents. Escherichia coli cloning vector pEASY-T1, expression vector pEASY-E2, competent cells Trans1-T1 and Transetta (DE3) were purchased from TransGen Biotech (Beijing, China). The whole gene of the mature form of ANGPTL8/betatrophin was synthesized by Inovogen Tech. Co. (Beijing, China) into … WebFigure S-1. The map and the construct of the pEASY-T3 cloning vector. 2. Affinity assay and aptasensing of the DNA aptamers to ProGRP31-98 The affinities of the aptamer A18, A18’, A18’’, and A18’’’ to ProGRP31-98 were investigated. The calibration plot of the FAM labeled A18 fluorescence intensity

拟南芥长链非编码RNA-DANA1及其在植物干旱耐受性的应用

WebThe pGEM®-T Easy Vector multiple cloning region is flanked by recognition sites for the restriction enzymes EcoRI, BstZI and NotI, thus providing three single-enzyme digestions for release of the insert. The pGEM®-T Vector cloning region is flanked by recognition sites for the enzyme BstZI. WebpEASY-Blunt3 (linearized) Cloning vector for blunt-ended PCR products. The cloned PCR product can be excised with EcoRI or NotI. Sequence Author: TransBionovo (TransGen Biotech) Open in SnapGene Try SnapGene for Free Download Plasmid Download SnapGene Viewer Explore Over 2.7kPlasmids:Basic Cloning Vectors More Plasmid Sets No matches med school personal statement template https://puremetalsdirect.com

pEASY CloNiNg VECtor - Biologica

Web您好！欢迎来到炼石商城 请登录 注册 我的订单; 我的炼石 WebThe pGEM®-T Easy Vector Systems are convenient systems to clone PCR products generated by certain thermostable polymerases. These polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments. The pGEM®-T Easy pre-linearized Vector contains 3´-T overhangs at the … WebThe “pEASY ” cloning vectors provide a highly efficient, 5 minutes, one-step cloning strategy for PCR product without the need of elimination of PCR buffers. Some of convenient features of the vectors are as follows. pEASY CloNiNg VECtor Fast Instead of traditional method----Ligation can be accomplished without T4 DNA ligase in as little as 5 med school personal statement word count

Combinatorial metabolic engineering of industrial Gluconobacter …

Category:pEASY®-T3 Cloning Kit 20 rxns - 炼石商城

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Peasy®-t3 cloning vector

Gastrin-Releasing Peptide (31-98), a Tumor Marker for Small …

WebpEASY-T3 Cloning Vector (TransGen Biotech, Beijing, China) and subsequently sequenced (Shanghai Sangon, Beijing, China). To obtain the complete cDNAs of Se-hsp90 and Se- hsp70 , respectively, gene-specific primers (hsp90G SPI, hsp90G SP2; hsp70G SPI, hsp70GSP2') were designed matching the primers in the 3' and 5' Full RACE kit (TAKARA). WebPeasy T3 Vector, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 86/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TransGen biotech co > peasy t3 vector. peasy t3 vector (TransGen biotech co) About; News;

Peasy®-t3 cloning vector

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WebMay 17, 2016 · The report gene gfp was amplified from the cloning vector pET28 (a)-GFP. The resulting product was digested with Xba I and Hind III, and then cloned into the Xba I and Hind III site of pBB5-P tufB , pBB5-P 0264 , pBB5-P 0452 and pBB5-P 0169 to generate the corresponding promoter strength reporter plasmids. WebEasyTaq PCR Supermix DNA聚合酶、pEASY-Blunt Simple Cloning Vector购自全式金公司；其他化学试剂购自Sigma公司。 2 结果与分析 2.1 拟南芥cpk6突变体纯合鉴定 为了获得纯合突变体，用SDS方法提取Col-0和cpk6幼嫩叶子的总DNA，利用T-DNA特异引物LBb1.3和CPK6基因特异引物LP和RP进行PCR ...

WebpEASY Cloning Vectors pEASY® -T1 Cloning Kit Catalog Number：CT101-01 Price：$89.02 Specification： 20 rxns 60 rxns Quantity： Manual Vector Information MSDS COA Inquire Now Buy Now Add to Cart Free Sample Avaliable Product Details pEASY® -T1 Cloning Kit is designed for cloning and sequencing Taq-amplified PCR products.

WebOct 1, 2016 · pEASY®- T3 Cloning Vector provides dual- Eco RI and dual- Not I restriction sites. It is designed for cloning and sequencing Taq- amplified PCR products 3’A … WebpEASY-T3 Cloning Kit. pEASY ®-T3 Cloning Vector provides dual-EcoR I and dual-Not I enzyme digestion sites. It is designed for cloning and sequencing Taq amplified PCR products. The cloned insert can be released from a single enzyme digestion. Cat. No. CT301. Specification: CT301-01: 20 rxns .

WebApr 1, 2016 · The plasmid pEASY-T3 cloning vector (TransGen Biotech, Beijing, China) was used as a TA cloning kit. The plasmid pGEX-4T-1 Vectors was used for prokaryotic expression. The structure maps of the pEASY-T3 and pGEX-4T-1 vectors are available online ( www.transgen.com.cn/ ).

Webp EASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of the pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing blunt-end PCR products. Suggested DNA Polymerases include TransStart ® FastPfu FLY and TransStart ® KD Plus Ultra-HiFi DNA Polymerases. Advantages of the pEASY®-Blunt Simple Cloning Kit nakie hammock coloursWeb7 rows · Product Details. pEASY® - T3 Cloning Vector provides dual EcoR I and dual Not I enzyme sites. It ... Products - pEASY® -T3 Cloning Kit - TransGen Biotech About TransGen - pEASY® -T3 Cloning Kit - TransGen Biotech Services & Support - pEASY® -T3 Cloning Kit - TransGen Biotech med school pharmacologyWebYN Zhang's Publication in Plos One....The RACE PCR products were subcloned into pEASY-T3 cloning vector system (TransGene, Beijing, China) and positive clones were sequenced by GenScript (Nanjing, China)... nakie thurman facebookhttp://www.lianshimall.com/goods-4341395.html med school phoenixWebA T7 RibomaxTM Express RNAi System (Promega) was used to prepare double-strand RNA (dsRNA), and pEASY-T3 vector (TransGen Biotech, Beijing, China) was used to clone the cDNA of green fluorescent protein (GFP) and Cs-Phm. T7 promoter sites were added by PCR using diluted plasmid templates and modified GSPs . Wizard HSV Gel (Promega) was … med school pharmacology study guide pdfWebA technology of bacillus and identification method, which is applied in the field of molecular biology and genetic engineering, can solve the problems of lack of theoretical basis, lack of identification method in the cultivation of salt-resistant crops, microbial fertilizers and plants that do not have efficient salt-alkali tolerance, and achieve saving The effect of … nakier maths a level praticeWebThree DXS genes with complete open reading frames was cloned into pEASY-T3 Vector, named PkDXS1, PkDXS2, and PkDXS3. The results of sequencing showed that PkDXS1, PkDXS2, and PkDXS3 had 2223 bp, 2217 bp, and 2142 bp, respectively. Bioinformatic Analysis of DXS from Simao Pine nakia resort and dive fiji